3MTJ
The Crystal Structure of a Homoserine Dehydrogenase from Thiobacillus denitrificans to 2.15A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 67.613, 67.613, 224.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.760 - 2.150 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25600 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.252 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.088 | 0.053 | 0.670 |
| Number of reflections | 29384 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 99.8 | 98 | 100 |
| Redundancy | 3.9 | 3.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.5M Ammonium sulfate, 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
