3MT9
Glycogen phosphorylase complexed with 4-nitrobenzaldehyde-4-(beta-D-glucopyranosyl)-thiosemicarbazone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2007-11-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.04498 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.270, 128.270, 115.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.690 - 2.050 |
| R-factor | 0.17503 |
| Rwork | 0.173 |
| R-free | 0.20498 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.147 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.093 | 0.450 |
| Number of reflections | 60721 | |
| <I/σ(I)> | 19.5 | 5.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 20 mg/ml of protein in a buffer solution containing 10 mM BES pH 6.7, 1 mM EDTA and 3 mM DTT. Crystals soaked with 6.7mM inhibitor in 20% DMSO for 3 hrs, SMALL TUBES, temperature 289K |
