3MT5

Crystal Structure of the Human BK Gating Apparatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-12
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	144.518, 144.518, 182.195
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.500 - 3.000
R-factor	0.248
Rwork	0.246
R-free	0.27800
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.215
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.089	0.032	0.738
Number of reflections	23149
<I/σ(I)>	9.4
Completeness [%]	99.9	99.8	99.9
Redundancy	5.8	5.2	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K