3MT0
The crystal structure of a functionally unknown protein PA1789 from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.749, 67.227, 48.446 |
| Unit cell angles | 90.00, 110.97, 90.00 |
Refinement procedure
| Resolution | 34.324 - 1.582 |
| R-factor | 0.1776 |
| Rwork | 0.176 |
| R-free | 0.20360 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.942 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.089 | 0.462 |
| Number of reflections | 34184 | |
| <I/σ(I)> | 28.5 | 2.45 |
| Completeness [%] | 97.6 | 92.9 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M MgCl2, 0.1M Tris, 20%(v/v) PEG8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
