3MSI

TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-07-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	33.300, 39.930, 44.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 1.430
R-factor	0.191 *
Rwork	0.214
R-free	0.25000 *
Starting model (for MR)	1msi
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	1.970
High resolution limit [Å]	1.430 *	1.900
Rmerge	0.065 *
Total number of observations	40197 *
Number of reflections	9650 *
<I/σ(I)>	7.8
Completeness [%]	83.9 *	98.3
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *	Jia, Z.,(1995) Protein Sci., 4, 1236. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3-3.5 (mg/ml)
2	1	drop	ammonium sulfate	25-27.5 (%sat)
3	1	drop	sodium acetate	0.05 (M)
4	1	reservoir	ammonium sulfate	50-55 (%sat)
5	1	reservoir	sodium acetate	0.1 (M)