3MQ6
Domain swapped SgrAI with DNA and calcium bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 130.384, 134.949, 237.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.456 - 2.000 |
| R-factor | 0.2118 |
| Rwork | 0.211 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.071 |
| Number of reflections | 266875 |
| Completeness [%] | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | PEG 4000, NACL, HEPES BUFFER, CACL2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | CACL2 | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | NACL | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | CACL2 |
