3MQ2
Crystal Structure of 16S rRNA Methyltranferase KamB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.302, 64.112, 72.069 |
| Unit cell angles | 90.00, 104.61, 90.00 |
Refinement procedure
| Resolution | 25.081 - 1.690 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.20970 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.106 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.050 | 0.285 |
| Number of reflections | 46638 | |
| <I/σ(I)> | 16.8 | 3.4 |
| Completeness [%] | 99.1 | 92 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.25M Potassium thiocyanate, 24% PEG 2000 monomethylether, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.25M Potassium thiocyanate, 24% PEG 2000 monomethylether, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
