3MQ0
Crystal Structure of Agobacterium tumefaciens repressor BlcR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00826 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.155, 74.773, 141.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.518 - 1.793 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.21830 |
| Structure solution method | SAD/SIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.037 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.081 | 0.389 |
| Number of reflections | 48827 | |
| <I/σ(I)> | 25.1 | 2.27 |
| Completeness [%] | 94.0 | 71.6 |
| Redundancy | 5.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 9% PEG400, 30 mM MgCl2, 30 mM MES, pH5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
