3MPX
Crystal structure of the DH and PH-1 domains of human FGD5
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97942, 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.140, 70.645, 127.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.710 - 2.800 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.560 | 2.800 |
| Rmerge | 0.142 | 0.071 | 0.469 |
| Number of reflections | 9116 | ||
| <I/σ(I)> | 5.5 | ||
| Completeness [%] | 99.8 | 100 | 96.9 |
| Redundancy | 6.9 | 6.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% w/v PEG 3350, 0.2M lithium sulfate, 0.1M HEPES pH 7.5. 1:100 trypsin was also added. vapor diffusion, sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP |
