3MPW
Structure of EUTM in 2-D protein membrane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.949, 149.069, 70.089 |
| Unit cell angles | 90.00, 120.01, 90.00 |
Refinement procedure
| Resolution | 46.980 - 2.700 |
| R-factor | 0.2525 |
| Rwork | 0.248 |
| R-free | 0.33641 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2A1B (residues 2-90) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.454 |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.980 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.032 |
| Number of reflections | 25180 |
| <I/σ(I)> | 19.31 |
| Completeness [%] | 84.1 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100 MM SODIUM DIHYDROGEN PHOSPHATE, 100 MM POTASSIUMDIHYDROGENPHOSPHATE, 2 M SODIUM CHLORIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
