3MPV
Structure of EUTL in the zinc-induced open form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979464 |
| Spacegroup name | P 3 |
| Unit cell lengths | 67.415, 67.415, 79.604 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.380 - 2.600 |
| R-factor | 0.22523 |
| Rwork | 0.222 |
| R-free | 0.26672 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gfh |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.171 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.430 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.117 |
| Number of reflections | 11274 |
| <I/σ(I)> | 1.9 |
| Completeness [%] | 98.9 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2 M NaCl, 100 mM phosphate, 100 mM MES buffer, 5 % PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
