3MPO
The crystal structure of a hydrolase from Lactobacillus brevis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.254, 108.254, 130.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.127 - 2.900 |
| R-factor | 0.2293 |
| Rwork | 0.226 |
| R-free | 0.28280 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.967 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.700 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.113 | 0.175 |
| Number of reflections | 28778 | |
| <I/σ(I)> | 17.8 | 10.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.9 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 4% Tacsimate pH 4.0, 12% w/v Polythylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
