3MPD
Crystal structure of nucleoside diphosphate kinase from encephalitozoon cuniculi, cubic form, apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9765 |
Spacegroup name | P 21 3 |
Unit cell lengths | 111.330, 111.330, 111.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.570 - 2.080 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1NSQ (Drosophila NDP kinase) |
RMSD bond length | 0.015 |
RMSD bond angle | 1.333 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.570 | 2.130 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.077 | 0.454 |
Number of reflections | 27311 | |
<I/σ(I)> | 20.88 | 5.7 |
Completeness [%] | 97.3 | 95.5 |
Redundancy | 11.4 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | PACT SCREEN CONDITION F8: 100MM BIS-TRIS PROPANE PH 6.5, 20% PEG 3350, 200 MM NA2SO4, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |