3MPC
The crystal structure of a Fn3-like protein from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-24 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.428, 45.731, 107.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| R-factor | 0.16743 |
| Rwork | 0.166 |
| R-free | 0.19624 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.980 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.860 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 23168 | |
| <I/σ(I)> | 24.87 | 7.16 |
| Completeness [%] | 96.8 | 94.8 |
| Redundancy | 5.12 | 2.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1.6 M ammonium sulphate, 0.1 M MES monohydrate, 10% v/v 1,4-dioxane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
