3MP2
Crystal structure of transmissible gastroenteritis virus papain-like protease 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.510, 62.510, 199.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.150 - 2.500 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.903 |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.159 | 0.778 |
| Number of reflections | 14461 | 3956 |
| <I/σ(I)> | 9.72 | 2 |
| Completeness [%] | 99.2 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | MES, ammonium sulphate, PEG 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
