3MP1

Complex structure of Sgf29 and trimethylated H3K4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-12
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	50.238, 84.845, 119.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 2.600
Rwork	0.223
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hsj
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.127	0.469
Number of reflections	15314
<I/σ(I)>	9.5	2.2
Completeness [%]	94.0	85.1
Redundancy	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	281	25% PEG3350, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K