3MOM
Structure of holo HasAp H32A mutant complexed with imidazole from Pseudomonas aeruginosa to 2.25A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 47.373, 46.378, 81.088 |
| Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
| Resolution | 24.640 - 2.250 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mok |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.617 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 4.840 | 2.250 |
| Rmerge | 0.116 | 0.056 | 0.535 |
| Number of reflections | 16389 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 96.6 | 97.2 | 91.8 |
| Redundancy | 2.9 | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% (w/v) PEG 8000, 100 mM imidazole, pH 8.0, vapor diffusion, hanging drop, temperature 293K |
