3MOL
Structure of dimeric holo HasAp H32A Mutant from Pseudomonas aeruginosa to 1.20A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 71.740, 52.700, 85.714 |
| Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
| Resolution | 19.245 - 1.200 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mok |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.041 | 0.350 |
| Number of reflections | 95175 | 19526 |
| <I/σ(I)> | 16.96 | 3.4 |
| Completeness [%] | 95.0 | 91.9 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.26 M ammonium sulfate, 100 mM HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 293K |
