3MOK
Structure of Apo HasAp from Pseudomonas aeruginosa to 1.55A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.984, 65.247, 38.343 |
| Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
| Resolution | 35.920 - 1.550 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ell |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.434 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.076 | 0.054 | 0.323 |
| Number of reflections | 21268 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 96.5 | 99 | 72.4 |
| Redundancy | 3.7 | 3.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 1.6 M NaH2PO4/0.4 M K2HPO4, 100 mM phosphate-citrate, pH 4.2, vapor diffusion, hanging drop, temperature 293K |
