3MO1
Crystal structure of the non-neutralizing HIV antibody 13H11 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.365, 60.436, 80.118 |
Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
Resolution | 40.050 - 1.800 |
R-factor | 0.1886 |
Rwork | 0.187 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mnv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.155 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_271)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.300 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.073 |
Number of reflections | 38807 |
<I/σ(I)> | 13.2 |
Completeness [%] | 96.6 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.2 | 298 | Reservoir: Qiagen AmSO4 suite condition with 2 M ammonium sulfate, 0.2 M ammonium acetate. Drop: 0.5 uL protein + 0.5 uL reservoir., pH 7.2, VAPOR DIFFUSION, temperature 298K |