3MN2
The crystal structure of a probable AraC family transcriptional regulator from Rhodopseudomonas palustris CGA009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 65 |
| Unit cell lengths | 64.097, 64.097, 88.088 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.981 - 1.800 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.24750 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.087 | 0.714 |
| Number of reflections | 18976 | |
| <I/σ(I)> | 42.8 | 3.2 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1M Phosphate-citrate 40% v/v PEG300, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
