3MN1
Crystal structure of probable yrbi family phosphatase from pseudomonas syringae pv.phaseolica 1448a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-03 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.965, 127.023, 107.418 |
| Unit cell angles | 90.00, 95.27, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.17688 |
| Rwork | 0.175 |
| R-free | 0.22182 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.850 | |
| Number of reflections | 190104 | |
| <I/σ(I)> | 6.8 | 0.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 100MM SODIUM CITRATE, PH 5.6, 30% PEG 4K, 200MM AMMONIUM ACETATE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
