3MN0
Introducing a 2-His-1-Glu Non-Heme Iron Center into Myoglobin confers Nitric Oxide Reductase activity: Cu(II)-CN-FeBMb(-His) form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Detector technology | CCD |
| Collection date | 2009-06-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0988 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.610, 48.420, 77.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.650 |
| Rwork | 0.245 |
| R-free | 0.27300 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.024 |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.063 |
| Number of reflections | 17410 |
| Completeness [%] | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | pH 7, EVAPORATION, temperature 298K |
