3MMS
Crystal structure of Streptococcus pneumoniae MTA/SAH nucleosidase in complex with 8-aminoadenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 145.720, 145.720, 145.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.600 |
| Rwork | 0.182 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.580 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.041 | 0.241 |
| Number of reflections | 34861 | |
| <I/σ(I)> | 13.6 | 2.8 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 17.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.26 M sodium citrate, 90 mM sodium HEPES, pH 7.5, 10 % (v/v) glycerol, vapor diffusion, hanging drop, temperature 298K |
