3MMP
Structure of the Qb replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 254.710, 139.400, 101.870 |
Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
Resolution | 47.283 - 2.500 |
R-factor | 0.2103 |
Rwork | 0.210 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.053 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.112 | 0.609 |
Number of reflections | 122568 | |
<I/σ(I)> | 18.29 | 2.87 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2M KCL, 0.05M Hepes pH 7.5, 27%-30% v/v pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, SITTING DROP, temperature 277K |