3MMK
The structural basis for partial redundancy in a class of transcription factors, the lim-homeodomain proteins, in neural cell type specification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.2819 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.823, 88.734, 49.851 |
| Unit cell angles | 90.00, 111.85, 90.00 |
Refinement procedure
| Resolution | 23.135 - 2.157 |
| R-factor | 0.2366 |
| Rwork | 0.235 |
| R-free | 0.26510 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.655 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.157 | 2.160 |
| Rmerge | 0.084 | 0.473 |
| Number of reflections | 20403 | |
| <I/σ(I)> | 14.269 | 2.458 |
| Completeness [%] | 98.1 | 78.2 |
| Redundancy | 6.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.5M magnesium sulphate, 0.1M MES, 3% (w/v) sucrose, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
