3MMI

Crystal structure of the globular tail of Myo4p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	43.476, 120.989, 157.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.510 - 2.300
R-factor	0.254
Rwork	0.254
R-free	0.28700
Structure solution method	SAD
RMSD bond length	0.030
RMSD bond angle	2.170
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.061	0.382
Number of reflections	35353
<I/σ(I)>	23.16	3.4
Completeness [%]	98.6	99.4
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	100mM HEPES, 20% PEG 3350, 150mM HCO2Na, 10 mM K2Pt(CN)4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K