3MMG
Crystal structure of tobacco vein mottling virus protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2007-06-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97939 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.476, 77.563, 78.463 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 1.700 |
R-factor | 0.17634 |
Rwork | 0.175 |
R-free | 0.20961 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q31 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.335 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.080 | 0.431 |
Number of reflections | 50064 | |
<I/σ(I)> | 17.4 | 2.7 |
Completeness [%] | 96.1 | 92.7 |
Redundancy | 5.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M potassium formate 20% PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |