3MME
Structure and functional dissection of PG16, an antibody with broad and potent neutralization of HIV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 191.961, 230.804, 82.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.450 - 3.970 |
| R-factor | 0.259 |
| Rwork | 0.256 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3LRS CHAIN AB |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.743 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.140 |
| High resolution limit [Å] | 3.970 | 4.000 |
| Rmerge | 0.237 | 0.556 |
| Number of reflections | 5511 | |
| <I/σ(I)> | 6.9 | 1.5 |
| Completeness [%] | 93.3 | 82.5 |
| Redundancy | 5.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | 1.96M ammonium sulfate, 0.2 M NaCl, 0.1M acetate pH 4.5 with 3% 1,6 diaminohexane, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
