3MM4
Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8423 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.458, 99.825, 79.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.000 |
| R-factor | 0.17852 |
| Rwork | 0.176 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AB5 (CheY) |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.254 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.000 | 2.043 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.296 |
| Number of reflections | 13674 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 98.3 | 75.9 |
| Redundancy | 11.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.05 | 293 | 0.1M MES, 2.54M Ammonium sulfate, pH 5.05, vapor diffusion, hanging drop, temperature 293K |
