3MLV
Crystal structure of anti-HIV-1 V3 Fab 2557 in complex with an NOF V3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.554, 42.745, 116.063 |
| Unit cell angles | 87.91, 85.22, 85.97 |
Refinement procedure
| Resolution | 35.540 - 2.480 |
| R-factor | 0.2387 |
| Rwork | 0.233 |
| R-free | 0.34190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.228 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.480 | 5.380 | 2.480 |
| Rmerge | 0.122 | 0.083 | 0.366 |
| Number of reflections | 25853 | ||
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 90.6 | 99 | 90.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 25% PEG 3350, 0.2 M MgCl2, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
