3MLU
Crystal structure of anti-HIV-1 V3 Fab 2557 in complex with a ZAM18 V3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.452, 43.167, 57.824 |
| Unit cell angles | 89.15, 86.25, 85.19 |
Refinement procedure
| Resolution | 35.167 - 2.770 |
| R-factor | 0.2017 |
| Rwork | 0.196 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.770 | 6.030 | 2.770 |
| Rmerge | 0.112 | 0.070 | 0.348 |
| Number of reflections | 10115 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 98.8 | 99.8 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 19% PEG 3350, 0.19 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
