3MLT
Crystal structure of anti-HIV-1 V3 Fab 2557 in complex with a UG1033 V3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.683, 142.927, 85.013 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 35.740 - 2.490 |
| R-factor | 0.206 |
| Rwork | 0.202 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 5.380 | 2.490 |
| Rmerge | 0.047 | 0.025 | 0.311 |
| Number of reflections | 62552 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 99.9 | 98.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 20% PEG 3350, 0.2 M Na formate, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
