3MKY

Structure of SopB(155-323)-18mer DNA complex, I23 form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	I 2 3
Unit cell lengths	200.780, 200.780, 200.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.490 - 2.860
R-factor	0.242
Rwork	0.242
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	141.000
High resolution limit [Å]	2.860
Rmerge	0.044
Number of reflections	30569
<I/σ(I)>	12.7
Completeness [%]	99.0
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	1.3 M ammonium sulphate, 0.1 M Tris pH 8.5 , 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K