3MKT
Structure of a Cation-bound Multidrug and Toxin Compound Extrusion (MATE) transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0093 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.840, 238.770, 45.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.770 - 3.650 |
| R-factor | 0.312 |
| Rwork | 0.312 |
| R-free | 0.34300 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.770 |
| High resolution limit [Å] | 3.650 |
| Number of reflections | 15364 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 295 | 50mM Tris-HCl pH 7.2-8.6, 87 mM (NH4)2SO4, and 16-24% polyethylene glycol 250 dimethyl ether in D2O, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
