3MKR

Crystal structure of yeast alpha/epsilon-COP subcomplex of the COPI vesicular coat

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792, 0.9794, 0.9642
Spacegroup name	I 4 2 2
Unit cell lengths	176.629, 176.629, 141.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.840 - 2.600
R-factor	0.222
Rwork	0.222
R-free	0.26300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.050	0.383
Number of reflections	34363
<I/σ(I)>	28.8	3.3
Completeness [%]	99.3	98.8
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	11% PEG 4000, 0.1M sodium citrate, 5mM DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	11% PEG 4000, 0.1M sodium citrate, 5mM DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K