3MKQ
Crystal structure of yeast alpha/betaprime-COP subcomplex of the COPI vesicular coat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790, 1.009 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 152.638, 152.638, 294.535 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.980 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.077 | 0.420 |
| Number of reflections | 133676 | |
| <I/σ(I)> | 19.6 | 2.8 |
| Completeness [%] | 96.3 | 91.6 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.8M potassium phosphate dibasic, 0.8M sodium phosphate monobasic, 0.1M Bis-Tris propane, 1% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
