3MKO
Crystal Structure of the Lymphocytic Choriomeningitis Virus Membrane Fusion Glycoprotein GP2 in its Postfusion Conformation
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-08-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.3354 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 52.925, 52.925, 191.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.220 - 1.800 |
| Rwork | 0.188 |
| R-free | 0.22220 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT (2.7.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.220 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.463 |
| Number of reflections | 13777 | |
| <I/σ(I)> | 26.9 | 2.5 |
| Completeness [%] | 89.9 | 88.2 |
| Redundancy | 13.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% MPD, 2% PEG 400, 100mM imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
