3MKL
Crystal structure of DNA-binding transcriptional dual regulator from Escherichia coli K-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.972, 81.856, 165.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.20846 |
| Rwork | 0.207 |
| R-free | 0.24681 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.124 | 0.403 |
| Number of reflections | 15608 | |
| <I/σ(I)> | 35.4 | 6.96 |
| Completeness [%] | 97.8 | 97.1 |
| Redundancy | 7.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 297 | 0.1M Citric acid pH 3.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
