3MKJ
Methionine gamma-lyase from Citrobacter freundii with pyridoximine-5'-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.843 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.690, 122.800, 128.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.905 - 1.650 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rfv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.049 | 0.302 |
| Number of reflections | 50624 | |
| <I/σ(I)> | 17.72 | 4.7 |
| Completeness [%] | 91.0 | 59 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 37% MMEPEG 2000, 200MM ammonium sulfate, 50MM TRIS-HCL, 0.2MM PLP, 0.2MM DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
