3MKH
Podospora anserina Nitroalkane Oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 43 |
| Unit cell lengths | 137.501, 137.501, 131.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.883 - 1.995 |
| R-factor | 0.1773 |
| Rwork | 0.176 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c0u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.217 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.995 | 1.995 |
| Number of reflections | 164769 | |
| <I/σ(I)> | 17.7 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.5 M magnesium sulfate, 0.1 M 2-(N-morpholino)ethanesulfonic acid, 18% glycerol, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
