3MK3
Crystal structure of Lumazine synthase from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 154.234, 151.503, 235.032 |
| Unit cell angles | 90.00, 97.08, 90.00 |
Refinement procedure
| Resolution | 32.029 - 3.569 |
| R-factor | 0.2261 |
| Rwork | 0.226 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rvv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.640 |
| High resolution limit [Å] | 3.569 | 3.580 |
| Rmerge | 0.091 | 0.557 |
| Number of reflections | 105485 | |
| <I/σ(I)> | 9.41 | 2.01 |
| Completeness [%] | 83.2 | 80.8 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6M Ammonium Sulfate, 100mM Tris buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
