3MJP
Crystal structure determination of Japanese quail (Coturnix coturnix japonica) hemoglobin at 2.76 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.090, 97.434, 61.705 |
| Unit cell angles | 90.00, 123.10, 90.00 |
Refinement procedure
| Resolution | 29.540 - 2.760 |
| R-factor | 0.2676 |
| Rwork | 0.265 |
| R-free | 0.28725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.862 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.760 |
| Number of reflections | 11820 | |
| <I/σ(I)> | 3.9 | 1.8 |
| Completeness [%] | 81.6 | 83.8 |
| Redundancy | 2.88 | 2.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 32% PEG 3350, 33mM Sodium Potassium phosphate buffer, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
