3MJO
Small subunit (R2F) of native ribonucleotide reductase from Corynebacterium ammoniagenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.210, 87.683, 83.249 |
| Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.360 |
| R-factor | 0.15 |
| Rwork | 0.150 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kgp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.073 | 0.477 |
| Number of reflections | 140012 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 96.1 | 72.7 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 27.5% PEG4000, 0.05M Ammonium acetate pH 6.0, 0.1 M ammonium acetate pH 7.0, 0.1 M Sodium citrate, 0.05 M Tris-HCL pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
