3MJN
Crystal Structure of the complex of C-lobe of lactoferrin with isopropylamino-3-(1-naphthyloxy)propan-2-ol at 2.38 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.107, 50.248, 65.654 |
| Unit cell angles | 90.00, 107.82, 90.00 |
Refinement procedure
| Resolution | 62.500 - 2.380 |
| R-factor | 0.17417 |
| Rwork | 0.171 |
| R-free | 0.21142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dwa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.428 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.500 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 15943 | |
| <I/σ(I)> | 9.1 | 1.9 |
| Completeness [%] | 97.4 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550, pH 6.5, VAPOR DIFFUSION, temperature 298K |
