3MJL
Crystal structure of human arginase I in complex with 2-aminoimidazole. Resolution 1.90 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 3 |
| Unit cell lengths | 90.680, 90.680, 69.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.168 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zav |
| RMSD bond length | 0.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.265 |
| Number of reflections | 50007 | |
| <I/σ(I)> | 13.9 | 4 |
| Completeness [%] | 99.1 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Drops containing 3uL of protein solution [3.5 mg/mL protein, 50 mM bicine (pH 8.5), 2 mM 2-aminoimidazole, 100uM MnCl2] and 3uL of precipitant solution [0.1 M HEPES (pH 7.0), 28% Jeffamine] were equilibrated against a 1 mL reservoir of precipitant solution., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
