3MJF
Phosphoribosylamine-glycine ligase from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.637, 69.151, 95.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.800 - 1.470 |
| R-factor | 0.137 |
| Rwork | 0.135 |
| R-free | 0.17400 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.740 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.097 | 0.883 |
| Number of reflections | 77813 | |
| <I/σ(I)> | 12.9 | 3.05 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 9.5 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate, 30% PEG-8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
