3MJB

Cricket Paralysis Virus IGR IRES Domain 3 RNA bound to sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Wavelength(s)	1.1271
Spacegroup name	P 41 21 2
Unit cell lengths	58.940, 58.940, 99.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.680 - 2.800
Rwork	0.242
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b31
RMSD bond length	0.006
RMSD bond angle	1.388
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	41.680
High resolution limit [Å]	2.200
Number of reflections	9451
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	1.4 M lithium sulfate, 42.5 mM magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH; transferred to saturated lithium sulfate, 42.5 mM magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH for cryo-protection, pH 7.5, VAPOR DIFFUSION, temperature 293K