3MJA

Cricket Paralysis Virus IGR IRES Domain 3 RNA bound to selenate, structure #2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Wavelength(s)	0.9786
Spacegroup name	P 41 21 2
Unit cell lengths	59.250, 59.250, 99.775
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.233
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 3B31
RMSD bond length	0.006
RMSD bond angle	1.381
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	42.000
High resolution limit [Å]	2.800
Number of reflections	8365
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	1.8 M lithium selenate, 42.5 mM magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH; moved to 2.4 M lithium selenate, 42.5 mM magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH (all otherfor cryoprotections, pH 7.5, VAPOR DIFFUSION, temperature 293K