3MJ3
Cricket Paralysis Virus IGR IRES Domain 3 RNA bound to selenate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Wavelength(s) | 0.9756 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.260, 59.260, 99.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| Rwork | 0.227 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b31 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.395 |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 3580 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 1.4 M lithium sulfate, 42.5 mM Magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH; transferred to saturated lithium selenate, 42.5 mM Magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH for cryo-protection, pH 7.5, VAPOR DIFFUSION, temperature 293K |
